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Determination of cyanocobalamin in stool Sample preparation Approximately, 20 mg of dry and pulverized stool samples were mixed with with 75 l of TCEP and 210 l of 1% formic acid in methanol

Results The enzyme samples are incubated in a phosphate buffer containing specific concentrations of glutathione (GSH) and peroxide and then incubated for a suitable time to react before Ellman's reagent (5,5-dithiobis-(2-nitrobenzoic acid) (DTNB) is added

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