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3 bed volumes of Equilibration Buffer (LE buffer) and 5 bed volumes of deionized water are used to equilibrate the resin in the order

155 Mechanistic studies have shown that RPSA, upon infection, undergoes Tyr204 phosphorylation and recruits the SNF2-related chromatin remodeling ATPase 5 (SMARCA5) subunit of the imitation switch (ISWI) complex, thus promoting NF-B binding to target gene promoters without altering other innate pathways

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doi: 10.1038/s41598-021-87619-8 11

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