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Furthermore, we determined the flux of glycolytic carbon through the pyruvate dehydrogenase complex (PDC) by stable-isotope labeling with 13 C-glucose

In solution, it produces a deep red color that can appear slightly more orange-tinted than cyanocobalamin, though this varies with concentration

Filtering removes the visible cloudiness by trapping aggregates, but you're left with a solution that contains far less peptide than the original concentration