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If late-stage secondary patents are now economically less valuable for small molecules, IP teams should concentrate prosecution resources on broader, earlier-stage patents that can be used to capture more commercial value during the pre-negotiation window, rather than filing dense thickets of late-stage formulation and method-of-use patents that will be economically devalued by the IRA clock

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Following incubation, the resin was washed thrice on a midi batch purification column (Generon, Maidenhead, UK) using wash buffer (20 mM Tris-HCl, pH 7.8, 0.5 M NaCl, 5 M urea, 10-50 mM imidazole) before elution in buffer containing 20 mM Tris-HCl, pH 7.8, 0.5 M NaCl, 300 mM imidazole and 5 M urea

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Researchers can explore additional educational content in the Frontier Peptide Labs Research Library, including articles covering peptide biology, analytical chemistry, molecular signaling, laboratory methodologies, peptide quality control, and current scientific research topics

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