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Suppression of Rab7a Expression 12 10 6 CHO M12 and SH-SY5Y cells were transfected with 100 nM ON-TARGET plus SMARTpool siRNA (Dharmacon) targeting human Rab7a mRNA (NM_004637) at position 480498 (5-CUAGAUAGCUGGAGAGAUG-3), 715733 (5-AAACGGAGGUGGAGCUGUA-3), 385353 (5-GAUGGUGGAUGACAGGCUA-3) and 243261 (5-GGGAAGACAUCACUCAUGA-3), together with Lipofectamine 2000 as described [39] After transfection, cells were grown in media containing 10% LPDS for 48 h, then loaded with 10% FCS 10 M (CHO M12) or 50 M (SH-SY5Y) drug candidate 10

In people over 60, the prevalence is around 2%.[ref] The lack of vitamin B12, though, can cause symptoms long before developing pernicious anemia

Fernandez-Hernando, C., Suarez, Y., Rayner, K

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