Stereological studies showed that the volume of testes, the length of seminiferous tubules, and the number of spermatogenic and none-spermatogenic cells decreased significantly during aging
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Quantitative real-time PCR (q-PCR) for mRNA level Total RNAs were extracted from MCF-7 and ZR-75-1 cells using RNA-easy Isolation Reagent (Vazyme, Nanjing, China) according to the manufacturers instructions
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