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Comparing with wild genotype (CC), the heterozygote (CT) and mutation homozygote (TT) lead to the decline of enzyme activity by about 34 and 75%, and increased thermolability in lymphocyte extracts 25
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Also, avoid rubber-closure vials, as they allow contamination through their hole and from rubber additives
10.1016/j.cub.2011.01.069 27
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