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Unlike injectable forms, the nasal spray facilitates the peptides delivery through the nasal mucosa, which may allow for quicker systemic distribution

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10.1080/01904161003728685 J

After five washing steps with PBS-Tween, the secondary antibody (Alexa Fluor 555 anti-mouse, Life Technologies, 1:200) was added in 2% BSA/PBS-Tween together with Hoechst 33342 (1 g/ml) for 2 h

This system uses selective digestion of sample DNA with methylation-sensitive and methylation-dependent restriction enzymes, followed by quantification of the remaining DNA using real-time PCR

Role of polyphenols and carotenoids in endothelial dysfunction: an overview from classic to innovative biomarkers