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Intracellular ROS levels were detected by incubating cells with 10 M CM-DCFDA for 30 minutes, then analyzed by Accuri-C6 flow cytometry

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Domestic studies usually use cell and molecular biology experimental methods to explore the effects of metformin on the glioma cell cycle and apoptosis, and the research results show that metformin can significantly change the cycle distribution of glioma cells ( Mitochondria-dependent apoptosis pathway: metformin can induce the loss of mitochondrial membrane potential and release apoptosis-related factors such as cytochrome C, thereby activating the caspase cascade and ultimately leading to apoptosis ( Inhibition of tumor cell metabolism: Metformin can reduce intracellular glucose levels and insulin signaling, thereby inhibiting the glycolysis process of tumor cells to further weaken the growth and proliferation of glioma cells

Takemura K, Board PG, Koga F

All deep-learning-based methods (CheMeleon, Egret-1 GNNs, XGBoost, and LightGBM) had very similar performance on this dataset, although ESOL-based models performed significantly worse