For measurement of metabolite levels in cells, hepatocytes were harvested by treating with 0.25 % trypsin (Gibco #25200), washing with ice-cold PBS to remove trypsin, suspending released hepatocytes in ice-cold PBS, and centrifuging the cell suspension at 1600 g for 10 min at 4 C to obtain the pelleted cells, which were immediately frozen and stored at 80 C for later analysis
Maricich SM, Wellnitz SA, Nelson AM, Lesniak DR, Gerling GJ, Lumpkin EA, et al
This cycle plays a key role in maintaining the intracellular reducing environment, protecting protein sulfhydryl groups, and regulating oxidative signaling by rapidly responding to changes in reactive oxygen species (ROS) levels
Garlic, shallots and onions are also rich in sulfur-containing compounds
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PMID: 29623453