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Solvent extraction followed by fractionation is employed to obtain the plant extract containing phytochemicals, with antioxidant, anti-inflammatory, and antimicrobial activity (Hamsa and Kuttan, 2011)

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Each reaction mixture was prepared using 10 l QuantiFast SYBR Green PCR master mix, 4 l of cDNA template with 1 M of each primer in a total reaction volume of 20 l