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Sulfate supply influences compartment specific glutathione metabolism and confers enhanced resistance to Tobacco mosaic virus during a hypersensitive response

Here are some tips to ensure a flawless application, no matter how you intend to get your glow on

10.1016/S0140-6736(17)30069-7 45 LeiterL

Cell viability and ROS and glutathione intracellular content of cells cultured in liquid medium were assessed by flow cytometry analysis of cells stained for 30 min in the dark with 0.025 M calcein-AM (ThermoFisher Scientific, C3099) at room temperature, 50 M Dichlorodihydrofluorescein-diacetate (DCFDA, ThermoFisher Scientific, D399) at 37 C, and 10 M monochlorobimane (ThermoFisher Scientific, M1381MP) at 37 C, respectively

The relevant OD value at 450nm for stopping the staining is between 2.0 - 2.5

Anal Lett 30(12):22112220 Rammesmayer G, Praznik W (1992) Fast and sensitive simultaneous staining method of Q-enzyme, -amylase, R-enzyme, phosphorylase and soluble starch synthase separated by starch: polyacrylamide gel electrophoresis