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Since vitamin D facilitates calcium absorption, there is a tendency that it will cause gastrointestinal distress and lead to a buildup of calcium
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Eluted protein was further purified with SEC (Superdex 75, Cytiva) in the SEC buffer (20 mM Hepes pH 7.5 and 400 mM NaCl)
doi: 10.3389/fnmol.2018.00140 166 AzpuruaJHineCVaidyaAMyakishev-RempelMAblaevaJMaoZet al
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