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High doses (above 12 grams daily) are more likely to cause gastrointestinal symptoms

10.1517/14656566.2012.656088 150 ReynoldsJ

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Western blotting Sample preparation Pulverized liver tissue (20 mg) was homogenized in ice cold 1x RIPA buffer (50 mM Tris, 150 mM NaCl, 1% NP40, 0.5% sodium deoxycholate and 0.1% sodium dodecyl sulfate (SDS), pH 8) using a glass dounce homogenizer