Finally, the samples were prepared for Western blotting analysis by lysing the brain homogenates on ice for 300 minutes in 100 mL of a lysis buffer composed of 120 mM NaCL, 40 mM Tris (pH 8), and 0.1% NP40, followed by centrifugation
Protocol Administer 1,200 mg IV slow push or diluted in 100 mL normal saline over 1530 minutes, once to twice weekly using a 2325G butterfly or IV cannula
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If a prescriber determines that BPC-157 therapy is warranted, starting at 150-200 mcg once daily with twice-weekly lab monitoring represents the most cautious approach
Theill LE, Karin M