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After removal of the overlying meninges and blood vessels, spinal cords were cut into small pieces, dissociated with 0.05% collagenase I (Thermo Fisher Scientific, Foster City, CA, USA) for 15 min at 37 C, and then processed to produce epSPC neurospheres as previously described 6,18
LCMV is a non-cytopathic virus that replicates slowly, which favors its persistence due to the failure of the host to mount an efficient cytotoxic T cell response 89,94
While these highly reactive species are essential for cellular survival, excessive amounts can inflict damage on various cellular organelles
Bacteriostatic water is a laboratory supply item used for the reconstitution of lyophilized research compounds
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