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If required, cells were transfected with plasmid DNA using jetPRIME according to the recommended protocol of the supplier, one to two days before imaging

DOI: 10.1016/j.molmet.2016.02.005

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They utilize osmotic pressure gradients generated by salt-based cores to drive drug release through semipermeable membranes, enabling precise kinetics independent of physiological conditions [48]

Limit alcohol: Cutting back on alcohol (or incorporating alcohol-free days throughout the week) can help reduce strain on your liver

If this occurs, slight dose adjustment (increasing by 50mcg if not already at 500mcg) may help