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Analytes (M-CuP or D-CuP) were diluted in PBST buffer (10 mM phosphate-buffered saline, 0.05% Tween-20, pH 7.4) and injected at increasing concentrations at a flow rate of 30 L/min, with 120 s association and 180 s dissociation phases

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At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S -transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta

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