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The lysosomal v-ATPase-Ragulator complex is a common activator for AMPK and mTORC1, acting as a switch between catabolism and anabolism

To achieve a higher throughput data collection, aberration-free image shift (AFIS) was used as implemented in the Thermo EPU software package

Healthcare and Laboratory Diagnostic Information

Bacterial titers were determined via plaque assays by serially diluting the bacteria in 12-well plates containing confluent Vero cells

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The experiments employed RAMP1 knockout (KO), RAMP3 KO, and RAMP1/3 double KO mice on a 129S2/SvEv genetic background