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In more serious cases they run the risk of splitting open and causing life-threatening haemorrhages, which in the 1970s was one of the most frequent reasons for emergency surgery

For RNA sequencing, total RNA was extracted from iPS cell-derived HLOs using an RNeasy Plus Mini kit (Qiagen) following the manufacturers protocol

Sections were washed three times with PBST, and DNA was stained with DAPI (1 g/mL

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