Further observations confirm these findings
Details of the antibodies and assay given in supporting information file
Additionally, Nampt knockout mice models demonstrated that NAD + depletion in muscle leads to progressive loss of muscle function, while NR treatment restored mitochondrial function and induced muscle remodeling and restoration 255
Samples were incubated overnight with a primary antibody against -MSH (Bioss, MA, USA, # BS-1848R) or MC5-R (Alomone Labs, Jerusalem, Israel, # AMR-025) followed by biotinylated horseradish peroxidase-conjugated secondary antibody incubation and detection with diaminobenzidine (ABC kit, Vector Labs, Burlingame, USA)
Danne T, Biester T, Kapitzke K, et al
[3] Naver S, Jimenez-Solem E, Christensen M, et al.Dulaglutide: a novel once-weekly glucagon-like peptide-1 receptor agonist[J].Clinical Investigation, 2014, 4(8): 729-743