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It depends on where you start from, how much support you need and receive, how you manage your taper and many other factors
Separated proteins were transferred onto Immobilon-P Transfer membranes (MilliporeSigma, IPVH00010), blocked for 1 h using 5% BSA in TBS0.5% Tween-20 and stained overnight with primary rabbit-anti-GCLC (Sigma-Aldrich, HPA036359) or affinity-purified rabbit-anti-GGT1 (GGT129, as previously described 63 ) 1:1,000 in BSA 2.5% overnight at 4 C
for example, it protects membranes by maintaining the reduced state of both -tocopherol and zeaxanthin, it prevents the oxidative denaturation of proteins under stress conditions by protecting their thiol groups, and it serves as a substrate for both glutathione peroxidase and glutathione S -transferase
G.DasanayakeA
10.1016/j.bbamem.2011.08.001 302