The enzyme GGT and hydrogen sulfide (H2S) speed up cell damage, harming organs and tissues
The assay buffer consisted of DMEM without phenol red (Gibco #11880-028) supplemented with 10 mM HEPES (Gibco #15630-056), 1X GlutaMAX (Gibco #35020-038), and 1% (w/v) ovalbumin (Sigma A5503), 0.1% (v/v) Pluronic F-68 (Gibco, #24040-032)
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Increased RGD uptake by tumor cells could potentially occur due to the binding affinity of RGD to integrins
To detect intracellular ROS and ferrous iron, ROS Assay Kit-High Sensitive DCFH-DA (DOJINDO) and FerroFarRed (Goryo Chemical, Inc.), were used, respectively, following the manufacturers protocol
Shiromizu, C