Briefly, 1 mL of assay mixture contains optimized concentrations of the following chemicals: 0,5 M K 2 HPO 4 (pH 7.0), 2,5 mM EDTA, 0,18 U/mL GR, 100 mM glutathione and 10 mM reduced NADPH and tissue extract (0,5 mL) was added in the spectrophotometer cuvette along with 0,1 mL of 60 mM cumene hydroperoxide, a suitable substrate for GP
Usage Cited in: EMBO J
Journal of Dairy Science, 105 (5), 37033715
Frequently asked questions How long does it take to see results from copper peptides
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Because the microgram quantities are so small, precise calculation is especially important: even small misreadings of the syringe correspond to proportionally large dose differences