CTK solution (0.25% trypsin + 1 mg/mL collagenase IV + 1 mM CaCl 2 + 20% KSR in DPBS ()) was prepared with trypsin ((2.5%), no phenol red, Thermo Fisher Scientific Inc.), collagenase (type IV, powder, Thermo Fisher Scientific Inc.), calcium chloride dihydrate (Fujifilm Wako Pure Chemical Corporation), Knock Out TM Serum Replacement (KSR, Thermo Fisher Scientific Inc.)
That means not everybody can reach sufficient levels by diet alone
This is easier to measure because the math is simpler, and the slightly larger injection volumes are still comfortable for subcutaneous administration
Table 2 summarizes key considerations for biocompatibility design across various organs, offering researchers targeted optimization strategies
Vascul Pharmacol.2013;59(3):76-82
PMID: 50 NagalingamAKuppusamyPSinghSVSharmaDSaxenaNK