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All qRT-PCR samples were run in technical duplicates and the Ct method based on the reference value estimate from 3 housekeeping gene (GAPDH, GUSB and TBP) was used to calculate relative expression level of each gene and reaction

Maintenance Phase : Once blood counts normalize, a dose of 1,000 mcg every month or every two to three months is standard for long-term health

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Room temperature is the documented storage condition, so travel at ambient temperature is acceptable as long as sustained heat exposure (hot car interiors, checked luggage in cargo holds during summer) is avoided

Move at least 1 inch from previous injection sites and use a systematic rotation pattern

This supports the methylation needed to build and repair the myelin sheath around nerve fibres