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GSH is synthesized exclusively in the cytosol in two consecutive ATP-requiring steps, the first of which, and rate limiting, being -glutamylcysteine formation by glutamate-cysteine ligase (GCL)

J Neurochem (1999) 73(3):130917.10.1046/j.1471-4159.1999.0731309.x 12 PerierCTieuKGuganCCaspersenCJackson-LewisVCarelliVet alComplex I deficiency primes Bax-dependent neuronal apoptosis through mitochondrial oxidative damage

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Glycine and glutamate are produced and used by many metabolic reactions and have relatively high cytosolic concentrations

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