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Transformants were plated on 7H10-ADC-Kan plates and recombinants were confirmed by PCR using specific primers (Supplementary Table 2 and Fig

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Because they are unable to get it

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DNA was not isolated from the first two samples collected in the LD/LD-V group, and the first sample collected in each of the other respective groups, due to the use of saline to flush the cecum (after weighing) during early collection (therefore, n = 25 cecal samples)

The practical tradeoffs between the two methods come down to time, customization, and cost