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Sequencing was performed using a HiSeq 2500 (Illumina), with a read length of 300 bp paired-end reads and at least 25,000 tags per sample

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1830 (5): p

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At the end point, cells were collected by trypsinization (the culture medium and PBS washes were collected and re-added to each sample) and washed once with assay buffer (10 mM HEPES, 140 mM NaCl and 3.3 mM CaCl 2 , pH 7.4)

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PLoS biology 18, e3000411 (2020)

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