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5-Amino-1MQ has a half-life of 46 hours, so NNMT inhibition returns to baseline within 2436 hours of the last dose

Modulation of chaperone function and cochaperone interaction by novobiocin in the C-terminal domain of Hsp90: evidence that coumarin antibiotics disrupt Hsp90 dimerization

doi: 10.1186/s13054-020-02925-3 36 Kutti SridharanG.KotagiriR.ChandiramaniV

Accurate measurement is vital for valid data