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Ice-cold MeOH (600 L), CHCl 3 (200 L), and H 2 O (500 L) samples were vortexed and then centrifuged (10,000 g , 10 min, 4 C) to precipitate proteins

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effects on clinical pregnancy and live birth rates were less consistent across treatments, although testosterone supplementation showed a statistically significant live-birth improvement (odds ratio 2.19, 95% CI 1.114.32)

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