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Critically, the GSH/GSSG redox couple maintains the thiol groups of structural and enzymatic proteins in their reduced, functional state preventing irreversible oxidative cross-linking that would impair protein function
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NAD+ powers energy and repair : Functions as cellular battery for energy production, DNA repair, and cognitive enhancement, with benefits lasting 24-72 hours via IV therapy
After 24 h, cells were transfected with 100 nM of Bcl-2 siRNA or control siRNA, using DharmaFect 1 transfection reagent (Dharmacon Inc, Chicago, IL, USA), according to the manufacturer's protocol
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