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In vitro studies commonly use GHK-Cu in the nanomolar to low micromolar range (1100 nM for signalling assays, up to 10 M for cell culture models)

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Several main mechanisms were suggested by which obesity mediates insulin resistance, including altered secretion patterns of adipocytokines, increased levels of glucocorticoids in visceral fat tissue, and increased secretion of pro-inflammatory agents [35, 37]

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