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Peptide bond hydrolysis occurs through a nucleophilic attack on the carbonyl carbon of the peptide backbonea reaction catalysed by water molecules and accelerated exponentially with temperature
The protein concentration was measured by BCA kit (Thermo Scientific TM Pierce TM BCA Protein Assay Kit, Waltham, MA, United States)
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doi:10.2147/cia.S118152 Rassaf T, Rammos C, Hendgen-Cotta UB, et al
Supporting insulin signaling at a low consistent level can improve energy, stabilize blood sugar, and reduce metabolic strain over time
