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Brain organoid tissue immunohistochemistry Organoid sections were washed in PBS for 5 min, permeabilized in 0.3% Triton-X in PBS for 5 min and incubated in blocking solution (10% normal goat serum, 1% bovine serum albumin, 0.3% Triton-X in PBS) for 30 min at RT

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Competing interests The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper