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Subsequently, cells were incubated with JC-1 dye for 2 hours

Targeting a 500 mcg dose: To administer 500 mcg, draw the solution to the 15-unit mark on the syringe (15 units x 33.33 mcg/unit = 500 mcg)

The effects of Tb4 are extensive

Br J Pharmacol 174(10):933949 Halls ML et al (2015) International union of basic and clinical pharmacology

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The tail suspension test as a model for assessing antidepressant activity: review of pharmacological and genetic studies in mice