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Epidermal cornification is preceded by the expression of a keratinocyte-specific set of pyroptosis-related genes
After five washing steps with PBS-Tween, the secondary antibody (Alexa Fluor 555 anti-mouse, Life Technologies, 1:200) was added in 2% BSA/PBS-Tween together with Hoechst 33342 (1 g/ml) for 2 h
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