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If the goal is to study the effects of elevated IGF-1, why not just administer IGF-1 directly
However, higher doses extend the duration of receptor occupancy and downstream signaling because more peptide molecules are available to bind receptors over the clearance window
That distinction matters more on GLP-1 medications than almost any other context
The activation of Akt by mTOR is essential for the phosphorylation of P70s6k, which plays a pivotal role in regulating the cell cycle and ensuring cell survival
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