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B Cell-Derived GABA Elicits IL-10+ Macrophages to Limit Anti-Tumour Immunity. Nature, vol

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In cells containing mitochondria, ALA is reduced in an NADH-dependent reaction with lipoamide dehydrogenase to form dihydrolipoic acid (DHLA), whereas in cells that lack mitochondria, ALA can instead be reduced to DHLA via NADPH with glutathione (GSH) and thioredoxin reductases (Jones et al., 2002)

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RNA Isolation Cells were washed with PBS without Ca 2+ and Mg 2+ (Sigma) and each sample (1 10 6 cells) was lyzed with 1 ml QIAzol (QIAGEN)

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