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Total RNA was extracted using TRIzol reagent and quantified with NanoDrop 2000

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The second pathway of GSH synthesis is that related to the recycling of cysteine

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Mol Pharmacol 46:909914 Kim SK, Woodcroft KJ, Khodadadeh SS, Novak RF (2004) Insulin signaling regulates gamma-glutamylcysteine ligase catalytic subunit expression in primary cultured rat hepatocytes

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Rajendran, R., Ren, M., Ning, P., et al., Promotion of athermogenesis by copper or ironwhich is more likely?, Biochem

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