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p53 TAD (13-61) was subcloned into the pET His 6 glutathione-S-transferase (GST) TEV LIC cloning vector (2G-T, obtained from Scotta Gradia, Addgene plasmid #29707) and transformed into BL21 (DE3) pLysS cells

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"Vitamin C ameliorates D-galactose-induced senescence in HEI-OC1 cells by inhibiting the ROS/NF-B pathway." Mol Biol Rep

Bibcode:2011ChEuJ..1710151H

Ceramide comforts and strengthens fragile skin barrier

However, excess selenium may contribute to oxidative stress rather than relieve it