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In vitro, DSIP has a half-life of approximately 15 minutes due to the action of a specific aminopeptidase-like enzyme

ab178860, Abcam) overnight at 4 C followed by incubation with a horseradish peroxidase-linked secondary antibody

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Furthermore, fertility testing of fluoxetine-treated male rats was not performed to directly assess its effects on reproductive function, and the study only employed animal models and did not explore the relevance of the findings for human patients