Arjinpathana N, Asawanonda P
Methionine synthase, a vitamin B 12 -dependent enzyme, converts homocysteine to methionine, regenerating tetrahydrofolate (THF) from 5-methylTHF
More recently, in CHD patients undergoing PCI, intravenous administration of probucol (500 mg twice daily, one day before and three days after surgery) increased serum GSH levels [244]
39 Similarly, lutein plus zeaxanthin, at doses of 10 mg and 2 mg, respectively, improved tests of memory, reasoning, and complex attention after one year in a placebo-controlled trial with 51 healthy subjects aged 1830 years
After protein quantification, loading buffer containing 10% SDS, 0.5% bromophenol blue in Tris-HCL (pH 6.8), and 10% -mercaptoethanol (M3148, Sigma) was added to each cell lysate and boiled at 95-100 C for 10 min
Available reducing agents include Sodium borohydride and sodium triacetoxyborohydride, among others