The Folin phenol reagent binds to the protein, resulting in a gradual reduction, indicated by a color change from yellow to blue
Additionally, the experiments were conducted in accordance with the Animal Research: Reporting of in Vivo Experiments (ARRIVE) guidelines [76] for the care and use of research animals
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doi: 10.3389/fimmu.2013.00470 54 De CandiaP.PrattichizzoF.GaravelliS.MatareseG
10.1183/09031936.02.00262402 Eur Respir J