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Endpoints frequently measured include the activity of alkaline phosphatase (ALP), an early marker of osteoblast differentiation, the expression of the master osteogenic transcription factor Runx2, and the deposition of a mineralized matrix, which can be visualized with Alizarin Red staining

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doi: 10.4161/23723556.2014.992217 155 SandhuRReinJDArcyMHerschkowitzJIHoadleyKATroesterMA

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Synergistic collagen production from both pathways