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However, because B12 is stored in our livers, it might take a while for these deficiencies to show (Glass, 1959)
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\Rapid Onset: Effects observed within ~30 minutes after subcutaneous administration
The commonly used method is high-performance liquid chromatography, supplemented by other validation methods, as detailed below
Researchers working with extended time courses should validate stability for their own assay conditions rather than assuming a fixed window
This strategy limits discomfort to the upper layers, while also promoting faster healing
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