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Freeze-thaw cycles and cold methanol/water were used to extract the intracellular metabolites from the quenched cell pellets according to protocols described by Smart et al
J.EdingtonD
There's no evidence of receptor downregulation like with many hormones, but cycling is standard practice for research peptides and helps maintain sensitivity
[65] Dopamine contributes to the action selection process in at least two important ways
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CHS: Chalcone synthase
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