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reliable systemic absorption Intravenous infusion Used in controlled pharmacokinetic characterization studies Intraperitoneal injection Common in rodent research protocols for systemic delivery Intranasal administration Investigated for GHRH analogs in sleep research applications Subcutaneous administration represents the most common research route due to ease of delivery, reproducible absorption kinetics, and sustained peptide exposure suitable for growth hormone secretion studies

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Finally, aliquot (3-L) of the deproteinized sample was analyzed by means of an ultra high-performance liquid chromatography triple quadrupole mass spectrometry (UHPLC-MS/MS)