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Once thawed: Centrifuge briefly (30 seconds at 10,000 rpm) to collect any condensation from the tube walls Prepare fresh dilutions in the desired experimental buffer (PBS, cell culture media, etc.) Maintain on ice during experiment setup Use within 24 hours of dilution
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In less critical scenarios, the safest approach is to wait for data
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Draw bacteriostatic water: Use a sterile syringe to withdraw the desired amount
812, www.ncbi.nlm.nih.gov/pmc/articles/PMC4684116/
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