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One of the biggest issues with developing diagnostic tools or targeted drugs is off-target effects, said Jefferson Chan, PhD, associate professor of chemistry at the University of Illinois Urbana-Champaign, who conducted the study with graduate student Melissa Lucero

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Finally, the pathway analysis was performed using ShinyGO v0.80 83 and Reactome 84 to investigate the enriched pathways associated with the significant proteins identified by the Random Forest method and Volcano plots

But they transformed a peptide with a 20-minute half-life into one that circulates for 20 to 30 hours

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